An efficient and simple HPLC method has been developed and validated for the determination of Dolutegravir in bulk and was applied on marketed Dolutegravir products. The mobile phase used for the chromatographic runs consisted of HPLC Grade Methanol and Acetonitrile in the ratio of 70: 30% v/v. The separation was achieved on a Symmetry C18, 250 mm x 4.6 mm i.d.5µm particle size column using isocratic mode. Drug peak were well separated and were detected by a PDA detector at 245 nm. The method was linear at the concentration range 6–14μg/ml for Dolutegravir. The method has been validated according to ICH guidelines with respect to system suitability, specificity, precision, accuracy and robustness. Dolutegravir limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.507μg/ml and 1.539μg/ml respectively. Results of analysis were validated statistically and by recovery studies.