Fish has an important role in food security and poverty alleviation in both rural and urban areas of Sudan, but little is known about the nutritive value of the Nile fish that are normally utilized either fresh or preserved dried, salted or smoked. The study was conducted to evaluate nutritive value of nile tilapia (Oreochromis niloticus) cultured in earthen ponds compared with concrete ponds in fish farms in Gezira State, Sudan. A total of 36 samples of nile tilapia (Oreochromis niloticus) were collected from earthen and concrete ponds fish farms around Gezira State and the samples were subjected to chemical analysis (moisture, dry matter, ash, crude protein, fat, and crude fibre). The data was subjected to SPSS by using completely randomized design (CRD). The findings of this study revealed that, Oreochromis niloticus from concrete ponds has higher level of nutritive value than earthen ponds farms except fibre contents is higher in earthen ponds farms than concrete ponds farms, and there was highly significant difference (P≤0.01). In nutritive value of Fish between two types of ponds system, except crude fibre is recorded significant difference at (P≤0.05) between two ponds culture system. According to the findings, we recommended that: Encouragements and attention should be paid to aquaculture sector in order to shift fish production gradually from fisheries sector to aquaculture systems.