Background: The incidence of glucose intolerance in Sudanese pregnant women was found to be within the universal estimates. Family history of diabetes is considered by many authors a risk factor for pregnancy glucose intolerance. Regarding the diversity of the Sudanese population, international screening and diagnosis criteria of glucose intolerance may not apply. Materials and Methods: This case-control cross-sectional study has recruited pregnant women at Dream Maternity Hospital (n=73) as well as nonpregnant women as control group (n=67). Blood samples were collected from all study units chosen by convenient sampling method and analyzed for blood glucose concentration using spectrophotometry and glucose oxidase-peroxidase kits. Results obtained were analyzed using SPSS (ANOVA). Results: Mean age in the pregnant women group was 29.8 years and in the control group was 25.4 years. 30.1% of the pregnant women were in their first trimester, 37% in their second trimester and 32.9% in their third trimester. No significant difference was noted when blood glucose levels were compared in both groups (p=0.461) and this was also the case when age was considered in this comparison (p=0.264). Positive family history for diabetes has shown an obvious effect on blood glucose levels (p=0.015). When trimester of pregnancy was taken into consideration still there was no significant difference (p=0.114) although those in their third trimester exhibited higher concentrations of blood glucose. Conclusion: Family history of diabetes is strongly related to high blood glucose levels in Sudanese pregnant women and warrants screening for GDM.