Abstract

A novel very rapid, sensitive, reverse phase High-Performance Liquid Chromatography (RP-HPLC) technique was developed for the quantitative simultaneous estimation of Memantine and Donepezil in bulk and pharmaceutical dosage form. It was resolved by using a mobile phase of Buffer, Methanol and Acetonitrile in the ratio of 65:35:10% v/v/v at a flow rate of 1.0 mL/min using UV-Visible detector at the wavelength of 234 nm for quantification. Efficient separation was achieved for Memantine and Donepezil on used Altima C18 (4.6mm × 150mm, 5.0 µm) Column. The retention times of Memantine and Donepezil were found to be 2.088 min and 6.068minutes respectively. The calibration graphs were linear and the method showed excellent recovery for Memantine and Donepezil respectively. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness. The results presented in this report revealed the development of simple, rapid, precise and accurate RP-HPLC method for immediate determination and validation of Memantine and Donepezil in bulk form and their marketed pharmaceutical dosage forms.