Abstract

This study aims to establish a cryopreservation procedure for early aging ovarian tissue using the vitrification method on animals, evaluating the survival rate of follicles after freezing and thawing as the effectiveness criterion. Cryopreservation techniques for ovarian tissue in animals have seen significant advancements in recent years. However, in Vietnam, there has been no research applying the vitrification method to ovarian tissue of prematurely aging animals. The choice of animal models is due to ethical constraints and limited access to human ovarian tissue. Animals share genetic and physiological characteristics with humans, making them an ideal model for studying ovarian aging. This study is expected to successfully establish a cryopreservation procedure for early aging ovarian tissue using the vitrification method on animals and evaluate the survival rate of follicles after freezing and thawing. The study is conducted on pig ovarian tissue using experimental methods. The ovarian tissue is divided into two groups: the control group (without cryopreservation) and the experimental group (cryopreserved using the slow freezing and vitrification methods). After freezing and thawing, the ovarian tissue is evaluated for survival rate, quality, and developmental potential of the follicles.