The Indian health-care facilities (HCFs) made some guidelines related to Cytotoxic drugs so called Cytotoxic policy for patient safety and health-care worker safety, and environmental monitoring program as per the available international guidelines.Carfilzomib is indicated for the treatment of patients with multiple myeloma and chemically it is a tetra peptide epoxy ketone and an analog of epoxomicin. Analytical method for the detection of Deactivating agent and concentration play an impartment role in the pharmaceuticals especially with cytotoxic molecules after completion of manufacturing and testing is mandatory to follow the safety protocol to dispose those materials. The present invention provides to identify the suitable deactivating agent for the neutralization of Carfilzomib injection 10 mg/mL and Carfilzomib API with respect to concentration and time. This method developed based by RP-HPLC.