Methicillin-Resistance Staphylococcus aureus (MRSA) is any strain of S. aureus that has developed resistance to beta-lactam antibiotics. Dogs are reported to be colonized with MRSA and may become potential transmission sources to other animal species and humans. This study aimed to detect methicillin-resistant Staphylococcus aureus (MRSA) in dogs brought to the Central Veterinary Hospital (CVH), Kathmandu, Nepal. This study was based on the cultural characteristics, morphology, biochemical, and drug sensitivity tests of the bacteria of interest. Nasal swabs from 450 dogs were taken at CVH, transported to the Central Veterinary Lab (CVL) in charcoal agar, and incubated in mannitol broth. Characterization, isolation, and identification of Staphylococcus aureus were performed in the Blood Agar, Nutrient Agar, and biochemical tests (catalase, coagulase, trehalose, and VP). Positive isolates were suspended in peptone water via standard methods. The antibiotic sensitivity test followed Kirby Bauer's disk diffusion method. MRSA was categorized on the basis zone of inhibition of the cefoxitin (CX 30) antibiotic (CLSI, 2012). The overall prevalence of MRSA in dogs was found to be 4%. The Prevalence of MRSA among males and females; and among various age groups were statistically non-significant (p>0.05). The prevalence of MRSA among the dogs with and without antibiotic courses was 4.1% and 3.9% respectively. No significant difference was found between the sensitivity of Cefoxitin, Ceftriaxone, Enrofloxacin, Erythromycin, Tetracycline, and Amoxicillin (p>0.05) antibiotics. The prevalence of MRSA in dogs registered at CVH is 4%. This indicates MRSA is an emerging pathogen and a threat to public health. Thus, it is necessary to evaluate MRSA before prescribing any antimicrobial agents to minimize the associated risk factors and concept for the development of appropriate strategies and judicious antibiotic therapy.