The Navigation District is an UPT in the field of navigation that is responsible for the implementation of navigation activities to realize and maintain ship safety in the waters and port land. Therefore, navigation services must be provided by officers who are competent and experienced enough to produce satisfactory performance. The purpose of this research is to determine the influence of competence and work experience of employees of Navigation District Type A Class II Tanjung Emas Port Semarang. The study was conducted using a quantitative method on the population of all employees of Navigation District Type A Class II Tanjung Emas Port Semarang with a selected sample of 100 people. The instrument used was a questionnaire for IBM SPSS 25 data collection to analyze the data. Data analysis was carried out using multiple linear regression analysis statistical tests, T test, F test and determination coefficient. The results showed that competence had a positive and significant effect on employee performance (β1 = 0.264; sig.= 0.000). Work experience had a positive and significant effect on employee performance (β2 = 0.211; sig. = 0.003). Simultaneously, competence and work experience have a significant simultaneous effect on employee performance (sig. = 0.000) with a large contribution 20,9% (R2 = 0,209).