Abstract

Objective: The objective of this research was to present the antimicrobial potential of AgNP’s synthesised from Aristolochia bracteolata leaves extract.

Methods: Evaluation of antimicrobial activity of the leaves extract was tested using agar well diffusion method along with the commercial control antibiotics (Ciprofloxacin) and the synthesised AgNPs were also tested against several microbial cultures (MTCC 10619, MTCC 0424, MTCC 872 & MTCC 1755). Further phytochemical analysis and characterisation of the synthesised nanoparticles was done to determine the shape and size of synthesised nanoparticles.

Results: Prepared methanolic extract of Aristolochia bracteolata showed significant antibacterial activity against chosen microbial cultures (Bacillus subtilis, Pseudomonas aeruginosa, Fusarium oxysporum and Aspergillus niger) showing gradual increase in inhibition zone as the extract concentration increased from 20µl to 80µl. Bacillus subtilis among the bacterial strain and Aspergillus niger among the chosen fungal strain showed the maximum inhibition at 80 µl 12.7 ±1.729 mm and 15.7 ±1.729 mm respectively. But in case of AgNP’s Bacillus subtilis showed 14 ±1.132 mm at 80µl as compared to Pseudomonas aeruginosa 8 ±1.132 mm at 80µl. In case of fungal strains Aspergillus niger showed max. Inhibition 15.7 ±1.729 mm for methanolic extract of leaves and for AgNP’s solution the max. Inhibition was showed by Fusarium oxysporum 9 ±1.132 mm at 80µl. In phytochemical analysis the presence of tannin and phenol was noted whereas alkaloids were absent. SEM revealed the smooth and spherical structure of the nanoparticles.

Conclusion: Aristolochia bracteolata-derived AgNP’s presented an effective approach for antimicrobial applications, demonstrating efficiency against a range of pathogens.