Fresh groundnut seeds were purchased at starlight market, Sabo along Ogbomoso-Ilorin road, Ogbomoso. The oil was extracted by pressing method and analyzed for proximate, vitamin, physicochemical and fatty acids compositions using standard methods of analysis.

The proximate analysis result revealed that crude fat was in abundance (98 ±0.12 %) while other nutrients were present in traces. Vitamin E was the highest vitamin followed by Thiamine (B1) and riboflavin (B2) with the following concentrations: 21.28±0.012 mg/100g, 17.16±0.008 mg/100g and 16.56±0.002 mg/100g respectively.

The physicochemical analysis revealed that saponification value was higher (142.4 ± 0.02 mgKOH/g) although below permissible level for vegetable oil followed by the iodine value (42.61±0.12 g/100g) and acid value (3.25 ±0.05 mgKOH/g) while peroxide value was 0.302± 0.02 meq/1000g.

The major fatty acids present were cis-9-hexadecenal (62.70%), 15- hydroxypentadecanoic acid (17.2%) and 9-octadecynoic acid (10.5%), while others were in traces.

The study has shown that the oil contained nutrients that make up balance diet and the vitamin E, B1 and B2 could make the oil a remedy for infertility and support body metabolism, therefore, the oil could be harmonized as food supplement, industrial raw material and medicine.