Abstract

In recent years, ZnO nanoparticles gained tremendous attention attributed to their unique properties. ZnO NPs exhibits antimicrobial properties. However, the properties of nanoparticles are depended upon their size and shape, which make them specific for various applications. The present study deals with the synthesis, characterization and evaluation of antibacterial potential of ZnO NPs synthesized by Vigna mungo and Rhizobacteria. The rhizobacteria have been isolated from the root nodule of V. mungo and has been morphologically, biochemically and molecularly characterized and identified to be Rhizobium sp. strain P4 and Bacillus flexus strain IFO15715. The GC-MS analysis of methanol leaf extract of V. mungo carried out for the detection and identification of bioactive compounds and this revealed phytol as the antibacterial compound while Squalene and Alpha – tocopherol have antioxidant and anti-tumor property. The antibacterial potential of ZnO nanoparticles and leaf extract of Vigna mungo were expressed by agar well diffusion assay. This method is well documented and standard zones of inhibition have been determined for susceptible and resistant values. The results showed both methanol extract and zinc oxide nanoparticles harbor significant antimicrobial activity on most of the tested organisms. The synthesized nanoparticles from Rhizobium sp. were characterized by analytical techniques like SEM, XRD, FTIR, and UV-Vis.