An experiment was conducted to determine the extent of genetic diversity among arabica coffee hybrid genotypes for quality characters at two different sites in South-western region of Ethiopia. Genetic diversity among eight Arabica coffee hybrids and two standard check for 11 quality (three green bean and eight organoleptic cup quality characters were studied across four environments. The randomized complete block design was used in each site (site-by-year combinations). The quantitative data were analyzed and the significance of effects declared using MIXED procedure of SAS and their pool data across genotypes were used for multivariate analysis. Genetic diversity of Hybrid coffee genotypes was assessed by multivariate analyses (Principal component, Genetic distance and Cluster). Variation for mean performance of coffee hybrids for all quality attributes was significant except aromatic intensity taste. The highest distance was formed between Hybrids HC4 and HC5 (8.56) while the least was formed between Hybrids HC2 and HC3 (0.93). CA and PCAbiplot classified the hybrids in to two main group. The role of aromatic quality, acidity, body, flavor and overall quality characters for the two vectors was positive across three axes (PC’s, 81.18% of variance) which is the indication of the important components of genetic divergence in these materials. Hybrid HC5 and variety check was discriminated for their lower green bean physical characteristics and high cup quality attributes (PC1, 65.56 % of variance) and hybrid HC4 for its opposite behavior (PC2, 15.62%) from other groups. Green bean physical quality characteristics were inversely related to organoleptic quality attributes in PCAbiplot indicating simultaneous gain in both characters becoming difficult. The study indicates the presence of low to moderate variation for coffee quality characters among the tested coffee genotypes.