The aim of this study was to evaluate the effect of water quality parameters on nutritive value of Catfish (Clarias gariepinus) in Khartoum State, Sudan. A total of 60 samples of catfish (Clarias gariepinus) were collected from Nile river and earthen ponds fish farms around Khartoum State and the samples were subjected to chemical composition analysis (Dry matter (DM%), Crude protein (CP%), Either Extract (EE%) and Crude fiber (CF%) moisture% Nitrogen Free Extract (N.F.E%) Ph as well as ash%). Also, a total of 36 samples of water were collected from the ponds where fish samples were taken, Water quality parameters (No2, No3, Cl2, PH, TDS, EC. and Salinity were done where fish samples has been collected. The data was subjected to SPSS by using one-way analysis of variance (ANOVA). The findings of this study revealed that, Catfish (C. Gariepinus) from Nile environment has Higher in nutritive values that catfish from earthen pond environments and there was highly significant difference (P≤0.01) except the pH. However, when compare Nile River water and earthen pond water, we found that; the pH and electrical conductivity showed a highly significant difference (P≤0.01), and total dissolved solids and salinity showed no significant difference (P>0.05). Accordingly, the study concluded that the fish from Nile River water has a high in nutritive values than earthen pond fish. Also, Nile river aquaculture environment is an immediate environment in relation to ponds environment. The study recommended that, aquaculture is so recent in Sudan and only few farmers are realized about water quality parameters, hence the facilities and equipment for physio chemical parameters measurements should be facilitated to aqua culturists and rearing fish.