Butternut Squash has various health benefits and high economic value. The cultivation of butternut squash in Pamekasan Regency is still largely dependent on the use of chemical fertilizers. The objective of this study is to examine the effect of organic fertilizers and NPK fertilizers on butternut squash, as well as to understand farmers' responses to the use of organic fertilizers in butternut squash cultivation. The research was conducted using an experimental method with a Randomized Block Design (RBD) to determine the impact of various organic and NPK fertilizers on the growth and yield of butternut squash. The results showed that the use of organic fertilizers and chemical NPK fertilizers significantly affected the growth response and yield of butternut squash. The best growth response and yield were observed in plants that received goat manure fertilizer at a dosage of 720 grams per plant.