Abstract

A simple, accurate, precise and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method has been developed, which can separate and quantitatively estimate Molnupiravir in bulk and marketed pharmaceutical dosage form. The chromatographic separation for Molnupiravir was achieved with mobile phase containing Acetonitrile and Phosphate buffer (0.01M, pH-3.2) in the ratio of 30:70% v/v and Symmetry C18 ODS (4.6mm × 250mm) 5µm particle size column in isocratic mode at room temperature and UV detection at 246nm. The compound was eluted at a flow rate of 1.0ml/min. The retention time of Molnupiravir were found to be 5.493min. The above method was validated in terms of system suitability, linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ) in accordance with ICH guidelines. The method was rapid, simple, economical and suitable for routine quality control analysis.