Abstract

Male infertility has started to increase very rapidly around the world. This increase in infertility comes with an increase in environmental pollutants and lifestyle factors, in addition to some genetic disorders in people living in Iraq. The aim of this research was to find out the causes of undiagnosed infertility and the extent to which genetic disorders affect infertility between genetic polymorphisms in the locus of two genes, namely, NRF2, and GST of both types (GSTT1, GSTM). In methods two distinct test tubes were used to collect semen and venous blood samples from each subject, one used for physiological analysis of semen and the other EDTA tube used for DNA extraction. Genomic DNA was isolated from blood from all samples from patients. The DNA concentration and purity were measured by Bio drop, and the DNA concentration was validated for the PCR reaction, using agarose gel electrophoresis, DNA quality was determined and stained with safe red DNA, at -20 °C, all samples were stored until further use. The current study showed a significant increase in physiological tests in infertile patients compared with the control group. In this study of the NRF2 gene, GST, the results of the study showed that all the samples that were studied from among the control group and male infertile males carry the heterogeneous genotype (AG) of the genetic mutation that affects the gene NRF2 at the locus rs35652124, and the distribution of the mutant genotype is (GG) and normal genotype (AA) were (0%), while the percentage of heterogeneous genotype (AG) appeared (100%). The study showed the polymorphism of the (GST) gene in terms of presence and deletion, and the results showed that there was a presence of the (GSTT1 and GSTM1) genes by 35%, a loss of the GSTT1 gene by 30% and a loss of the GSTM1 gene by 35% in infertile men compared to the control group. The presence of high genes (GSTT1 and 1GSTM) was 55% and loss of GSTT1 gene and low GSTM1 gene was 25% and 20%, respectively, compared to the control group