Enhancement synthesis of nucleic acid, protein and chlorophyll in callus of mustard Brassica juncea L treatment with Laser
Abstract
This study deal with the effect of diode laser on embryonic and hypocotyls callus of the brown mustard Brassica juncea L. Embryo callus produced on the agar solidified medium (Murashige and Skoog,1962) MS + 0.3 mg /l-1 α-Naphthylacetic acid (NAA)+ 1.5 mg /l-16-Benzylaminopurine (BAP). Where hypocotyls callus was produced on (Murashige and Skoog, 1962) MS + 0.2 mg /l-1 α-Naphthylacetic acid (NAA) + 0.5 mg /l-1 6-Benzylaminopurine (BAP). Laser diode at a wavelength of 650 nm and a power of 50 milliwatts / cm2 for durations of 10, 20, 30, 40 seconds was used to expose at a distance of seven cm from laser source. rnData indicates that laser treatment enhancement the nucleic acid DNA and RNA concentrations. Moreover protein and chlorophyll content was increased in both types of callus.
How to Cite This Article
Jamal Ahmed Al Zobedy, Jamella Hazza Rasheed (2021). Enhancement synthesis of nucleic acid, protein and chlorophyll in callus of mustard Brassica juncea L treatment with Laser. International Journal of Multidisciplinary Research and Growth Evaluation (IJMRGE), 2(6), 38-43.