Abstract

The results of the research showed the isolation and diagnosis of Pseudomonas aeruginosa bacteria from a woman with urinary tract infection, and she reviewer for Al-Khansa Teaching Hospital. The bacterial isolation was diagnosed based on microscopic diagnosis, phenotypic characteristics and biochemical tests, as well as molecular diagnosis was made through PCR technology and based on the 16S rRNA gene as well as Matching the sequences of the genes with the sequences documented in the National Center for Biotechnology Information (NCBI). A new gene was obtained in the bacterium Pseudomonas aeruginosa in addition to being registered as Pseudomonas aeruginosa ZH-Almola in the (NCBI).rnThen, a sensitivity test was conducted for the most commonly used antibiotics for urinary tract infections, 10 types of antibiotics were used, and Ciprofloxacin was the most effective antibiotic.rnImportant virulence factors were also investigated, as five virulence factors were studied (hemolysin enzyme, urease enzyme, protease enzyme, the ability to form biofilms, and the ability to produce beta-lactamase enzymes), The results showed their ability to produce most of the important virulence factors, as the bacteria was a producer of hemolysin enzyme and urease enzyme and capable of forming biofilms and beta-lactamase enzymes, while it was not producing protease.