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1. Statistical processing and graphic generation were performed using Python scripts, prioritizing reproducibility and efficient resource usage: International Journal of Multidisciplinary Research and Growth Evaluation www. allmultidisciplinaryjournal. com 443 | P a g e Matplotlib: rendering of high-resolution scientific graphs. Tkinter: graphical interface. Native structures: use of dictionaries and lists for lightweight matrix manipulation.
2. 2.5. Ethical aspects For the conduct of the research, informed consent was taken into account, as well as the ethical standards that made it possible to promote and ensure respect for all participants in the study, so that their criteria/opinions and individual rights were respected, in order to generate new knowledge without violating the ethical principles of privacy and confidentiality of personal information [51] .
3. 3. Results and Discussion Regarding the municipalities with the highest concentration, Santa Clara stands out as the most prevalent (Figure 2), a result that agrees with previous studies on the matter [9,52,53] . It is followed in order by the municipalities of Placetas, Sagua la Grande, Manicaragua, and Caibarién, which largely coincides with the results obtained in research carried out in this province in previous years [9,41,50] , with only slight changes in the position/location of the municipalities of Manicaragua, Ranchuelo, and Caibarién, where the latter now stands out among the five municipalities with the highest concentration, which was not the case in the historical chronology of this province for Ae. aegypti. Fig 2: Results of the municipalities with the greatest focus during the study period in the province of Villa Clara A clearer view of the above is shown in figure 2, which reveals that the municipality of Santa Clara had the highest concentration of cases during the six years of the study, with 2022 being the year with the highest concentration, followed by 2023, and then 2020 and 2021, respectively (Figure 3). These results are consistent with those obtained in previous years in the same province [9,41,54] . It is important to note that this higher concentration in the municipality of Santa Clara is related to its status as an urban area with a higher population density and a deficient supply of potable water. This necessitates the use of numerous and varied water storage containers in homes. Therefore, it is reasonable to consider that urbanization positively impacts the number of reported cases, a finding consistent with results obtained by other authors, both in Cuba and in other countries of the Americas [3,23,41,55] . Fig 3: Distribution of Ae. aegypti focus by municipalities during the six years of study International Journal of Multidisciplinary Research and Growth Evaluation www. allmultidisciplinaryjournal. com 444 | P a g e In relation with the evolution of monthly focality (Figure 4), the months of June stand out in the following order, followed by November, July, October, September and August; it is worth noting that four of the months with reports of greater focality are located within the rainy period for Cuba, which corresponds to some extent with results obtained in previous years, both for this province, as well as in other localities of the country [9,41,53,55,56] , with 2022 being the year with the highest sustained focality for months during the six years that the study covered. Fig 4: Evolution of focality by month during the six years covered by the study All of the above is closely related to climatic variables, especially temperature, precipitation, humidity, atmospheric pressure, and wind speed, as demonstrated in previous studies conducted in this province [9,41,50,56] . The marked influence of temperature on mosquito breeding sites, particularly for Ae. aegypti (Figure 5), is noteworthy, as it manifests in the shortening of the life cycle. Temperature acts as a catalyst for this cycle, with sustained temperatures above 30°C shortening it from 9-12 days to as little as five days [9,41,50] . Furthermore, there is a marked correlation between the epidemic outbreaks of Dengue and the seasonal periods established for Cuba, since it is precisely in the months of June, November, July, September and October, where the largest epidemic outbreaks of this infectious entity occur, with the occurrence of said outbreaks being more likely in the last four months of the year [50,53,55,56] , something very similar occurred in Cuba for the emerging entity Chikungunya (CHIKV), which broke out last year as an epidemic outbreak and with an exponential growth throughout the year. Fig 5: Map with color contrasts for monthly heat and its relationship with the focus on Ae. aegypti during the six years of the study Figure 6 shows the total annual trend in terms of the focality of Ae. aegypti in the province of Villa Clara during the six years covered by the study, showing a downward trend of this variable, which is not a true reflection of the reality in these last four years, after the COVID-19 pandemic, but we can assert that these results confirm the seasonal cyclical behavior of the culicids [9,41,56] . International Journal of Multidisciplinary Research and Growth Evaluation www. allmultidisciplinaryjournal. com 445 | P a g e Fig 6: Total annual trend of Ae. aegypti focus in Villa Clara province during the six years of the study
4. 4. Conclusion The overall annual trend in terms of the focality of Ae. aegypti in the province of Villa Clara during the six years covered by the study was a decrease in this variable; where it is evident that the arboviral infectious entities have a close relationship with climatic seasonality, which confirms the seasonal cyclical behavior of the culicids.
5. 4.1. Transparency The authors confirm that the manuscript is an honest, accurate, and transparent account of the study, and that any iscrepancies from the study as planned have been explained.
6. 4.2. Competing Interests All authors contributed equally to the conception and design of the study. All authors have read and agreed to the published version of the manuscript. References
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