The present study was focused on formulating and evaluating Cisplatin containing niosomes formulation for invitro studies. Niosomal formulations were prepared by using different ratio of surfactant (Tween 80 and Span 80) and cholesterol by thin film hydration method and were evaluated for in vitro characteristics, stability studies. Span 80 containing niosomal formulation displayed highest entrapment efficiency with desired particle size. SEM analyses showed that niosomal formulation was spherical in shape. Niosomes containing Span 80 displayed higher percentage of drug release after 8h as compared to other formulations. F-4 formulation was found to be stable at the end of the study on storage condition. The present study suggested that niosomal formulations provide sustained and prolonged delivery of drug with enhance bioavailability.