The characterization of non-treated liquid waste is essential in designing an effective waste management system. This study explores the profile of wastewater contamination from Slaughterhouses based on the regulation of the Minister of Environment No. 5 of 2014. In addition, this study analyzed the fluctuation of Chemical Oxygen Demand (COD) for 14 days with treatment variations of P0 to P5. Laboratory testing at Trunojoyo University Madura showed a very high level of organic contamination The BOD reached 246.66 mg/L, exceeding the 100 mg/L threshold. COD of 286.93 mg/L also exceeded the 200 mg/L threshold. During testing, the COD degradation response showed statistically significant variation in the initial (days 0 and 3) and late (day 14) phases. However, there was no significant variation in the intermediate phase (days 5 and 7). P2 treatment on day three showed the best results with the lowest COD of 119.57 mg/L. This signifies the elimination of reactive simple organic compounds. In the intermediate phase, stagnation occurs due to difficulties in the biotransformation of complex compounds into intermediate products. In the final phase, P0 treatment resulted in the most optimal reduction of COD to 116.33 mg/L, indicating system stabilization. Other treatments, namely P1, P2, P3, and P5, experienced persistent organic compound accumulation with a fixed COD above 270 mg/L. This study concluded that the effectiveness of the treatment in reducing COD was influenced by the structural complexity of the pollutant and the process of re-release of dissolved metabolites in the system. This study confirms that waste management approaches require attention to temporal dynamics and the composition of pollutants.