In Chile, the Canine Herpes Virus has been detected by classical virology techniques such as isolation in cell cultures and subsequent cytolysis of affected cells; by immunofluorescence; by the presence of inclusion bodies or by viral kinetic studies which has made it possible to complete the biological analysis of a national isolate called RP5. In contrast, in the present work, the molecular detection of the ul37 gene of CaHV-1 was carried out, a gene that codes for the viral UL37 protein.rnThe visualization of the amplicon was carried out by electrophoresis in agarose gel at 2% at 90 volts for 90 minutes. After electrophoresis, the gel was incubated in ethidium bromide, subsequently washed, placed in an ultraviolet light transilluminator, and finally photographed. All the cell culture supernatants used were positive for the described PCR, observing a band of around 500 bp, which agrees with what is described in the literature.rnThe amplified product was purified, sequenced and the percentage of nucleotide identity (PNI) was determined by BLAST program. Thus, it was determined an NIP>97 and strongly suggests that the sequence obtained it belongs to CaHV-1.rnThus, we can conclude that this methodology allows the detection of a DNA amplicon with characteristics compatible with those described for the CaHV-1, and the ul37 gene as target using a molecular tool that meets the characteristics of current diagnosis: fast, sensitive, specific and low cost.rnThis method undoubtedly contributes to solving an existing problem in canine breeding farms, by having the possibility of offering the detection of CaHV-1 in small animals as companion pets, an area of sustained growth.