This article presents the simulation of the bioconversion of syngas produced during natural gas gasification into biodiesel compatible lipids, and compares it to the bio-GTL process to the Fischer-Tropsch synthesis routes in terms of product quality, emission levels, exergy, and energy efficiencies using Aspen Hysys simulation Software. The direct use of natural gas is limited by infrastructure changes necessitated by its use in transportation. The Fischer–Tropsch process, or indirect conversion of natural gas to liquid fuels (GTL), is a complementary strategy (synthesis of syngas or methanol). These liquid fuels and their intermediates have been found to be inefficient and energy-intensive in industrial settings where they are currently manufactured. Bioconversions consume less energy, cost less, and are more product-specific than chemical conversions. The method consisted of two steps: anaerobic syngas conversion to acetic acid via the acetogen Moorella thermoacetica, and aerobic acetic acid conversion to lipids via Yarrowia lipolytica, a genetically engineered oleaginous yeast. With an overall productivity of 34.47 percent and a carbon conversion efficiency of 18.60 percent, the integrated continuous bench-scale reactor system produced 36 percent of C16-C18 triacylglycerides directly from synthesis gas. Technically, the newly developed Bio-Gas to Liquid (GTL) pathway is feasible and has the potential to outperform the traditional Fischer–Tropsch process.