The Research work was designed to study and compare the physico-chemical Characteristics of milk samples of two different species like Damani goat and sheep. 300 Milk samples were collected from the Damani Goat and Sheep maintained at Livestock Research and development station Paharpur, Dera Ismail khan and its surroundings areas. The milk samples were analyzed for different physio-chemical parameters, including pH, specific gravity, titratable acidity, total solids, ash, fat, protein and lactose. It was recorded that Damani Goat milk had 0.17±0.01% titratable acidity, 1.030±0.001 specific gravity, 6.52±0.07 pH, 12.86±0.57% total solids, 0.76±0.14% ash, 3.98±0.52% fat, 3.17±0.33% protein and 4.37±0.34% lactose. Damani Sheep milk contained 0.23±0.01% titratable acidity, 1.035±0.002 specific gravity, 6.62±0.05 pH, 18.16±0.22% total solids, 6.50±0.24% fat, 5.32±0.30% protein, and 4.74±0.31% lactose and 0.89±0.07% ash. All the parameters tested were higher in Damani Sheep milk to Damani Goat milk.